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C15440-20UP

The ORCA‑Fusion BT camera represents the pinnacle of scientific CMOS (sCMOS) performance. Its specifications are uncompromising: ultra‑low readout noise, CCD‑level uniformity, high frame rates, and a back‑illuminated design that delivers exceptional quantum efficiency. Yet the true strength of the ORCA‑Fusion BT lies in what this extraordinary combination of photon detection and acquisition enables: revealing even the faintest signals, capturing visually stunning, high‑S/N images from the tiniest number of photons, resolving previously unattainable temporal events, and confidently executing advanced computational methods. The ORCA‑Fusion BT simplifies experimentation, making possible what was once thought impossible.

Classification：

Scientific camera

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Technical Data

Advantages of the ORCA-Fusion BT Compared to Other BT CMOS Cameras

Industry-leading, proprietary sCMOS performance, backed by trusted Hamamatsu quality and reliability.

  • Extremely low readout noise
  • High QE, broad wavelength range
  • Uniformity comparable to that of a CCD; lowest high readout noise per pixel
  • High Dynamic Range
  • 3 types of readout speeds
  • Light-sheet readout mode
  • 5.3 megapixels
  • USB 3.0 and high-speed CoaXPress interface

Advantages of ORCA-Fusion BT Compared to EM-CCD Cameras

A practical and powerful alternative that perfectly replaces costly EM-CCDs, offering outstanding sensitivity, speed, and versatility.

  • Resolution
  • Pixel Merge Versatility
  • No amplification or excess noise
  • High Dynamic Range
  • Wide field of view

Purpose

  • Wide-field fluorescence microscope
  • Time-lapse imaging
  • Confocal spinning-disk microscope
  • Light-sheet microscopy
  • Ultra-high-resolution precise positioning
  • Genetically Encoded Voltage Imaging
  • Optogenetics
  • Single-molecule tracking
  • Computational imaging
  • Quantum computing

Time-lapse live-cell imaging via low-light excitation

Sample: H9c2, Objective: Apo TIRF 60XC (NA 1.49), ND: 8, Interval: 15 seconds

Cell nucleus

c15440-20up Application Example 1

Nucleus:
HCS NuclearMask Stain/Exposure 20 ms

 

Mitochondrion

c15440-20up Application Example 3

Mitochondria:
MitoTracker/Exposure 500 ms

 

thin film

c15440-20up Application Example 2

Thin film:
CellMask Plasma Membrane Stain / Exposure 500 ms

Light-sheet readout mode [Patent]

“Light-sheet readout mode” is a unique patented feature of Hamamatsu sCMOS cameras that enhances the signal-to-noise ratio in light-sheet microscopy.
For more information on the principles and characteristics of the light-sheet readout mode, please refer to the details below.

Publication

ORCA-Fusion BT
Author Title Source
Pratish Thakore, Michael G Alvarado, Sher Ali, Amreen Mughal, Paulo W Pires, Evan Yamasaki, Harry AT Pritchard, Brant E Isakson, Cam Ha T Tran, Scott Earley Brain endothelial cell TRPA1 channels initiate neurovascular coupling Electronic Life, 10, e63040 (2021).
Lexy von Diezmann and Ofer Rog Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad J. Phys. Chem. B 2021, 125, 23, 6162–6170
Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis Nature Communications, 12, 7145 (2021).
Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy, and Kimani C. Toussaint, Space-time vector light sheets Optics Express, 29, 37225–37233 (2021)
Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine, Markus Missler, Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior Cell Reports, Volume 35, Issue 11, 2021, 109266,
Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae G3 Gene | Genome | Genetics, December 2021, Issue 12, Volume 11, jkab304

 

PC Recommendations

With the introduction of ORCA‑Fusion BT, users can now stream 5‑megapixel images to their computers at 89 frames per second. By following the guidelines outlined in the ORCA‑Fusion BT PC Recommendations, you can meet your computer’s requirements for high data rates.

Software

Software

Through the interface provided by our software, you can access all of our meticulously designed camera features, ranging from simple exposure settings to orchestrating complex triggers for multidimensional experiments.

Product Number C15440-20UP
Imaging equipment Scientific CMOS image sensor
Effective pixel count 2304 (H) × 2304 (V)
Pixel size 6.5 μm × 6.5 μm
Effective area 14.976 mm (H) × 14.976 mm (V)
Maximum well capacity 15,000 electrons *1
Readout speed Fast scan, full resolution, CoaXPress: 89.1 frames/sec
Fast scanning, full resolution, USB 3.0: 31.6 frames/sec (16-bit), 42.2 frames/sec (12-bit), 63.3 frames/sec (8-bit)
Standard scan, full resolution, CoaXPress: 23.2 frames per second
Standard scan, full resolution, USB 3.0: 23.2 frames per second
Ultra-quiet scanning, full resolution, CoaXPress: 5.42 frames per second
Ultra-quiet scanning, full resolution, USB 3.0: 5.42 frames per second
Readout noise Fast scan: 1.6 electron rms *1
Standard scan: 1.0 electron rms *1
Ultra-quiet scanning: 0.7 electrons rms *1
Exposure time Fast scan: 17 μs to 10 s
Standard scan: 65 μs to 10 s
Ultra-quiet scanning: 280 μs to 10 s
Cooling method Peltier cooling
Cooling temperature Forced air cooling (ambient temperature: +25 °C): −8 °C
Water cooling (water temperature: +25 °C): −8 °C
Water cooling (maximum cooling capacity, ambient temperature: +20 °C, water temperature: +20 °C): below −15 °C
Dark current 1.0 electrons/pixel/second (cooling temperature: −8 °C, ambient temperature: +25 °C) *1*3
1.0 electrons/pixel/second (cooling temperature: −8 °C, water temperature: +25 °C) *1*3
0.7 electrons/pixel/second (cooling temperature: below −15 °C, ambient temperature: +20 °C, water temperature: +20 °C) *1*3
Dynamic range 21 400:1 *1*2*4
External Trigger Mode Edge, level, synchronous readout, start, global reset edge, global reset level, programmable
External Trigger Signal Routing SMA
External Trigger Delay Function 0 μs to 10 s (in 1 μs increments)
Trigger Output Global exposure timing output, trigger-ready output, low-level output, high-level output, and three programmable timing outputs.
External Signal Output Routing SMA
Interface CoaXPress (dual CXP-6) and USB 3.0 SuperSpeed *5
Analog-to-digital converter 16-bit, 12-bit, 8-bit
Lens interface Type-C interface (standard), Type-F interface (C15440-20UP01)
Power supply AC 100 V to AC 240 V, 50 Hz/60 Hz
Electrical power consumption Approx. 155 VA
Operating ambient temperature 0 °C to +40 °C
Storage ambient temperature -10 ℃ to +50 ℃
Operating environment humidity 30% to 80% (non-condensing)
Storage environment humidity 90% or lower (no condensation)

*1 Typical value

*2 Calculated based on the ratio of maximum阱 capacity to RMS read noise.

*3 Dark current depends on the cooling temperature.

*4 In ultra-quiet scanning mode

*5 Equivalent to USB 3.1 Gen 1 (SuperSpeed USB 5 Gbps)

 

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