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Multiphoton apochromatic objective lens
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Technical Data
Multiphoton apochromatic objective with correction collar
● Dry objectives ideally suited for two-photon imaging
● Correction ring, used for imaging samples in various media
● M32 x 0.75 thread
The TL10X-2P and TL15X-2P plan‑field, extended‑apochromatic objectives combine the diffraction‑limited axial chromatic aberration performance of our other objectives in the visible spectral range with a high transmission spanning up to 1300 nm, making them ideally suited for multiphoton imaging applications (see…). Curve Tags). The TL28X-MP plan-apochromatic objective is optimized for the near-infrared and delivers diffraction-limited performance across the 900–1650 nm wavelength range, making it an excellent choice for multiphoton microscopy—including three-photon excitation. For more information on how these objective grades are defined, please refer to Objective Lens Tutorial Tag.
The 10× objective combines a numerical aperture (NA) of 0.50 with a long working distance of 7.77 mm, providing ample space for sample‑manipulation equipment. The 15× objective features an even higher NA of 0.70, optimized for collecting two-photon fluorescence signals, with a working distance of 2.6 mm. The 28× objective boasts the highest NA of 1.10, delivering enhanced sensitivity, and has a working distance of 2.8 mm. A correction collar on each objective allows adjustment of spherical aberration introduced when imaging through aqueous solutions or thick coverslips (see Correction ring Tags). The TL15X-2P objective also features a locking mechanism that secures the position of the correction collar, enhancing repeatability. The TL28X-MP objective is designed for water‑immersion applications. Because the front element of this lens is concave, use a pipette or syringe to introduce water into the front of the objective, ensuring no air bubbles form.
Please note that, for optimal performance, the front focal plane of the tube lens in your system should be positioned at the objective’s aperture diaphragm. For many objectives, this diaphragm is located at the shoulder of the objective. The aperture diaphragms of the TL10X‑2P, TL15X‑2P, and TL28X‑MP objectives are situated 42.0 mm, 52.2 mm, and 68.7 mm from their respective shoulders. If the diaphragm is not fully illuminated, vignetting may occur. For example, the focal plane of Thorlabs’ TTL200MP tube lens is 228 mm from the tube lens shoulder. Consequently, the spacing between the tube lens’s front element and the shoulders of the TL10X‑2P, TL15X‑2P, and TL28X‑MP objectives should be 186 mm, 175.8 mm, and 159.3 mm, respectively. To view these examples, click the blue information icon () in the “Alignment Diagrams” column of Table G3.3 to access the alignment diagrams.
Due to the large outer diameter of this objective lens housing, it may not fit properly with mounting brackets featuring an M32 × 0.75 internal threaded recess, such as models like… CSN200 and CSN210 . In this case, M32M32S The brass microscope adapter features both an M32 × 0.75 external thread and an internal thread, providing additional clearance between the objective lens housing and the mounting base.
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